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. 2015 Jul 10;10(7):e0132697. doi: 10.1371/journal.pone.0132697

Fig 9. Apoptotic erythroid cells at different stages of maturation in bone marrow and spleen.

Fig 9

Mice were given cadmium chloride dissolved in drinking water (50 ppm and 1000 ppm). After 5 weeks of exposure mice were sacrificed and their bone marrow and spleen cells were harvested. Cells thus isolated were stained with anti-mouse CD71-FITC, anti-mouse Ter119-APC and Annexin-V-PE. Different maturational stages of erythropoiesis were determined by relative Ter119/CD71/FSC expression and the apoptotic cells in each of these stages was estimated by Annexin-V staining. Extent of apoptosis in the total erythroid population (panel A) and at different maturational stages of erythroid differentiation in bone marrow (panel B) and spleen (panel C) is shown above. Each bar in the graph represents mean ± SEM of observations on 5 mice. *p<0.05, **p<0.01, ***p<0.005 and ****p<0.001 for comparison of the groups. Statistical analysis was done using Student t-test.