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. 2015 Jul 10;10(7):e0131908. doi: 10.1371/journal.pone.0131908

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Fig 3 — (A) Examples of scatter plots from fluorescence-activated cell sorting (FACS) of GFP+ cells from the indicated Tg(GBT-B4)fcc lines. Plots show GFP versus forward scatter (FSC, indicates cell size). The AB wildtype is a negative control. The line name and percent positive GFP cells out of the total events is indicated for each plot. (B) RT-PCR analysis of igH-μ, lck and ß-actin in GFP⁺ cells from the indicated Tg(GBT-B4)fcc lines. Lanes lacking an actin signal are not shown; vertical separation indicates independent experiments. Duplicates of fcc301 are shown (B-C). (C) Quantification of igH-μ and lck expression in GFP⁺ cells from the indicated lines. A.U. = arbitrary units. The scoring scale is listed below the results.