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. 2015 Jul 10;10(7):e0132604. doi: 10.1371/journal.pone.0132604

Fig 4. LDDN-0075935 treatment did not attenuate active, phosphorylated Src, Lyn, ERK, JNK, or p38 protein levels in BV2 cells.

Fig 4

Microglial BV2 cells were treated with vehicle (DMSO), 0.5nM, 5nM, 50nM, 0.5 μM, 5μM, and 50 μM LDDN-0075935 for 1h. Cells lysates were used for western-blot analyses with (B) anti-pLyn (Tyr 396), (C) anti-pSrc (Tyr 416), (D) anti-pERK, (E) anti-pJNK, and (F) anti-p-p38 antibodies with Lyn, Src, ERK2, JNK, and p38 antibodies as their respective loading controls. Optical densities from three independent experiments were graphed and averaged ± SD (*p<0.05vs. vehicle). (A) A representative western blot is shown.