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. 2015 Jul 10;10(7):e0132934. doi: 10.1371/journal.pone.0132934

Fig 2. Inhibition of PI3-Kinase alters choline uptake in SH-SY5Y cells.

Fig 2

Cells were treated with the PI3-Kinase inhibitor LY294002 with the concentrations indicated in the Fig Panel A. HC-3-sensitive choline uptake by CHT was measured as indicated in Fig 1 following 5 min exposure of differentiated SY5T-CHT cells to LY294002 treatment. Data are normalized to the control treatment group for n = 3 independent experiments. Panels B-D. Cells were prepared for immunoblot analysis of pPKB/Akt, CHT and actin levels following 5 min treatment with LY294002 from 4 independent experiments. Panel B. Densitometric analysis of pPKB levels were normalized to sample actin levels. Panel C. Densitometric analysis of CHT protein levels were normalized to sample actin levels. Panel D. The representative immunoblots shown are from 4 independent experiments. Data in Panels A to C are expressed as the mean ± SEM, with statistically-significant differences assessed by repeated-measures ANOVA with asterisks denoting p < 0.05.