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. 2015 Jul 10;10(7):e0132566. doi: 10.1371/journal.pone.0132566

Fig 4. Altered expression of miRNAs in mESCs by RA treatment.

Fig 4

(A) Scatter plot of the miRNA expression differences between DMSO- and RA-treated samples. (B) Scatter plot of the predicted novel miRNA expression between DMSO- and RA-treated cells showing differentially expressed miRNAs in the two samples. (C) RA downregulated the expression of miRNAs associated with pluripotency of mESCs, a finding validated by qPCR. mESCs were treated with 1 μM RA or DMSO for 24 h. (D) qPCR determined that RA upregulated the expression of differentiation-associated miRNAs. Representative miRNAs that were downregulated after treatment with 1 μM RA for 24 h. (E, F, and G) MiR-200a, miR-200b, and miR-200c relative expression levels after transfection with the expression vector PCDH-miR-200b or PCDH-miR-200c for 48 h. (H) qPCR revealed enhanced Oct4 and Nanog expression levels after transfection with the expression vectors PCDH-miR200a, PCDH-miR200b, or PCDH-miR200c for 48 h (without RA). Data are presented as the mean ± SEM of three independent experiments (*p < 0.05; **p < 0.01).