Fig 2. Major mucus components—Agr2, Fcgbp, Klk1, Muc2 and Zg16 –are unaltered in Clca1 ^-/-colonic mucus compared to WT controls.

(A) Scatter plot depicting correlation in the median label-free intensity of all mucus proteins between WT and Clca1 ^-/- mice by proteome analysis using mass spectrometry with the correlation coefficient (Pearson’s r) and r² shown. Proteins marked with red represent known major mucus components (Agr2, Fcgbp, Klk1, Muc2 and Zg16). Line = x + 1; n = 5 per group. (B) IF of Agr2, Fcgbp, Klk1 or Zg16 (red) in WT and Clca1 ^-/- distal colon sections, confirmed that there is no difference in protein expression in any of the major mucus proteins in Clca1 ^-/- mice compared to WT, n = 2–3 per group. Blue = Hoechst 34580 DNA-stain, scale bars 100 μm.