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. Author manuscript; available in PMC: 2015 Jul 10.
Published in final edited form as: Mol Cell. 2007 Jul 20;27(2):262–274. doi: 10.1016/j.molcel.2007.06.027

Figure 1. A Method for the Analysis of Protein Complexes in Human Cells.

Figure 1

(A) Flow chart of the procedure used for the affinity purification and mass spectrometry identification of protein complexes in HEK293 cells.

(B) Computational validation of protein interactions. Sensitivity/specificity ROC curve, parameterized by the IR score, for the discrimination between literature-supported interactions and dubious interactions (see Table S1). The chosen IR score threshold results in a sensitivity of 83% and a specificity of 83%. The IR was calculated based on the method described in the Supplemental Experimental Procedures.

(C) Summary of the overall screen for protein interactions.

(D) List of the 32 TAP-tagged polypeptides used in this study.