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. Author manuscript; available in PMC: 2015 Jul 10.
Published in final edited form as: Mol Cell. 2007 Jul 20;27(2):262–274. doi: 10.1016/j.molcel.2007.06.027

Figure 4. A Complex Comprising the Elongation Factor P-TEFb, the 7SK and U6 snRNAs, and the Previously Uncharacterized Protein BCDIN3.

Figure 4

(A) Network highlighting the interactions of BCDIN3 and CDK9 with various RNA processing factors, P-TEFb (cyclin T1/cyclin T2 subunits), and its regulatory factors HEXIM1 and HEXIM2.

(B) Gel filtration analysis showing that P-TEFb, HEXIM1, and the RNA processing factor SART3 cofractionate with BCDIN3.

(C) The 7SK and U6 snRNAs are present in the BCDIN3-TAP eluate. RNA blots were performed on total RNA extracted from HeLa cells (T; 900 ng) or RNA extracted from the BCDIN3-TAP eluate (B; 60 ng) and probed with RNA oligos specific for U2, U6, or 7SK snRNAs. Migration of the three intact RNA species is indicated.

(D) The 7SK snRNP complex is partly resistant to extensive digestion with RNase A. SDS gel showing BCDIN3 affinity eluates prepared from cell extracts treated or not with RNase A. Western blots confirming the presence of BCDIN3, P-TEFb, HEXIM1, and SART3 in both eluates are shown.