Fig 5. Effect of oral administration of fish oil or soybean oil containing DHA and/or EPA alone at daytime under free-feeding conditions on phase shifts and insulin level.

(A) magnitude of phase shifts induced by tuna oil or soybean oil containing DHA and/or EPA administration at ZT0 for 2 days under free-feeding conditions. Tuna oil, soybean oil, DHA- or EPA-containing oil was administered at 0.034 ml/10 g BW for 2 days, and mice were sacrificed to examine the phase of bioluminescence of liver on Day 3. (B) tuna oil, menhaden oil, or DHA-containing oil was administered at 0.034 ml/10 g BW at ZT0 under free-feeding conditions, and mice were sacrificed 2 h after injection to examine serum insulin level. *P < 0.05 (vs. SB group, Tukey-Kramer test). Numbers in parentheses indicate the number of tested mice.