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. 2008 Jul 8;13(9a):2843–2852. doi: 10.1111/j.1582-4934.2008.00406.x

Figure 1.

Figure 1

Immunoblot analysis of chondrosarcoma cell lines upon the overexpression of CDKN2A/p16 (A) or knock-down of CDK4 (E). (B) The characteristic band shift of pRb on 7.5% gel in the chondrosarcoma cell lines SW1353 and OUMS27. VH10 that was density-arrested and serum starved and subsequently reseeded in 20% serum (+) served as a control for hyper-phosporylated pRb. (F) The decreased ability of OUMS27 to form colonies after knockdown of CDK4. Relative cell numbers (C, G) and cell viability (D, H) compared with controls are shown (both measured 6 days after treatment). *significant differences.