Figure 5.
Results of mRNA amplified by real-time PCR. Total RNA was purified from SCL-1 cells treated with 10−5 M acitretin for 0, 6, 12, 24, 36 and 48 hrs. Two micrograms of total RNA were reversely transcribed and the levels of Fas, FasL and β-actin mRNA were determined by real-time PCR. A relative quantification analysis (two standard curves method) was carried out. The ratio of a target DNA sequence to a reference β-actin sequence in samples from untreated cells was served as a ‘calibrator’. Figures are representative of three independent experiments.