Figure 6.
Effects of acitretin on the expression of Fas, FasL and FADD proteins in SCL-1 cells. Cells were treated with 10−5 M acitretin for the indicated times, and then the cells were harvested. 20 μg cell extracts were resolved by 7.5% SDS-polyacrylamide denaturing gels and analysed for Fas, FasL, FADD by Western blotting with anti-Fas, FasL and FADD antibodies. As control, antibodies to β-actin were used for loading equal amount of specimens. The experiment shown is representative of three independent experiments showing similar results.