Extended Data Figure 8. Gene expression of TR1, TR1^exT_H17 and TH17 cells.

a, Heat map of genes selectively expressed in both TR1^exT_H17 and TR1 compared to TH17 cells. The bioinformatics analysis is based on the genes listed in Supplementary Table 1. Red squares highlight genes linked to TGF-β1 signalling. b, Relative mRNA expression of the indicated genes in TR1, TR1^exT_H17 and TH17 cells FACS sorted form the intestine of Fate⁺ mice treated with anti-CD3 monoclonal antibody is shown. Values shown are relative to TH17 cell gene expression. Mean and s.e.m. of biological independent experiments (IRF8 n = 2; SMAD3 n = 4; FOXO1 n = 2; STAT5a n = 3; SMAD4 n = 4) except for IL-23 and Runx1 (n = 2 technical replicates) are shown. In each experiment we pooled intestinal lymphocytes isolated from 7 treated mice before FACS sorting.