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Specific activities across various concentrations of enzyme. A) LST B) LytO-LBD C) SsaALP-LBD. ΔMFI s-1 (change in mean fluorescence intensity per second) represents the slope of the steepest linear region of the lysis curve. Each data point represents the mean of duplicate measures (LST) or quadruplicate measures (LytO-LBD and SsaALP-LBD) from a representative experiment, and error bars are standard deviations. Biological replicates exhibited the same trends.
