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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Ocul Surf. 2015 May 18;13(3):187–203. doi: 10.1016/j.jtos.2015.05.002

Figure 4.

Figure 4

In vivo CMTF data collected from mouse using the modified HRT-RCM system. A. Normal mouse cornea (C57/BL6). B. The wound healing response 7 days after transcorneal freeze injury (FI). C. The wound healing response 7 days after mechanical debridement (MD) injury with basement membrane removal. Mice were anesthetized using ketamine/xylazine for both procedures. For FI, a 1 mm diameter stainless steel probe cooled with liquid nitrogen was applied to the anterior, central corneal surface two times for 10 seconds each. For MD, the cornea was marked using a 2 mm diameter skin biopsy punch. The epithelium and basement membrane were then removed using an Algerbrush as previously described.52 A relative estimate of stromal cell and ECM backscattering was obtained by measuring the area under the CMTF curve as previously described (shaded areas under curves, “area” on top right of each image). Note the increase in backscattering following injury.