FIG. 7.

Analysis of exported DNA library samples from the microfluidic chip, with post assembly PCR. Lanes (1) 1b2b3a4a, (2) 2b3a3b4b, (3) 1a1b4a4b, (4) 2b3a4a4b (5) 1b2a3a4b, (6) 1b3b4a4b, (7) 1a2b3b4a, (8) 2b3a4a4b, (9) 1b2a3a4a, (10) 1b2b3a4b, (11) 1a2a3b4b, (12) 1b2a2b4b, (13) 2b3a4a4b, (14) 1a1b2b3a, (15) 1a2b3b4b, and (16) 2a2b4a4b. Apart from case (9), which exhibits only a very weak band, all assemblies containing each of 1-4 components were successful, as confirmed in separate experiments by sequencing (see text).