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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Nat Protoc. 2015 Jun 11;10(7):985–1006. doi: 10.1038/nprot.2015.065

Figure 2. Overview of ReN VM cell 3D culture protocol.

Figure 2

The experimental procedure begins with the generation of human neural progenitor cell lines virally transfected with APP and/or PSEN1 FAD mutations and enriched based on GFP and/or mCherry signals by fluorescence-activated cell sorting (FACS). The right-hand column shows in detail the timing for each step of the 3D culture method. Day 0 indicates the day on which cells are mixed with Matrigel. MAP2, microtubule associated protein 2; 4R tau, 4-repeat tau isoform; vGluT1, Vesicular glutamate transporter 1; NR2B, N-methyl D- aspartate receptor subtype 2B; TH, Tyrosine Hydroxylase.