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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Nat Protoc. 2015 Jun 11;10(7):985–1006. doi: 10.1038/nprot.2015.065

Figure 3. FACS (Fluorescence-activated cell sorting) enrichment of ReN-G and -GA cells for higher expressions of APP with FAD mutations.

Figure 3

a. The transfected ReN cells stably expressing GFP alone (ReN-G) were enriched by FACS based on the GFP signal intensity. Rectangular boxes indicate the cell populations that were selected/enriched for the experiments. ReN-G50 or 50, ReN-G cells with the top 50% GFP signal intensity; ReN-G10 or 10, ReN-G cells with the top 10%; ReN-G2 or 2, ReN-G cells with the top 2%; fsc, forward scatter which is roughly size/diameter; ssc, side scatter which is granularity. b. The transfected ReN cells stably expressing APPSL/GFP (ReN-GA) were enriched by FACS based on the GFP signal intensity. ReN-GA50 or 50, ReN-GA cells with the top 50% GFP signal intensity; ReN-GA10 or 10, ReN-GA cells with the top 10%; ReN-GA2 or 2, ReN-GA cells with the top 2%. c. Representative confocal fluorescence images showing ReN-G and ReN-GA cells with the top 2% (Ren-G2 and Ren-GA2), 10% (Ren-GA10) and 50% (ReN-GA50) GFP signal intensity. Green, GFP; Scale bar, 25 μm. d. The confocal fluorescence microscope images of enriched ReN-G2, -GA2 cells that were differentiated by growth-factor deprivation for 4 weeks (Green, GFP; Scale bar, 25 μm).