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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Nat Protoc. 2015 Jun 11;10(7):985–1006. doi: 10.1038/nprot.2015.065

Figure 4. Various 3D culture formats.

Figure 4

a. A 24-well plate with inserts yields the thick-layer cultures ideal for molecular, biochemical, and ELISA analyses. b–d. For thin culture, the 96-well plate (b), 35 mm dishes (c), and 8- or 16-chambered cover glass compartments (d) offer glass-bottom options that facilitate high-resolution immunofluorescence staining.