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. 2015 May 15;43(12):5759–5770. doi: 10.1093/nar/gkv498

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — HMO1 eviction from the vicinity of DSB. (A) ChIP showing HMO1 localization at indicated loci relative to the DSB site during continuous damage induced by galactose. IC, input control; No, no antibody; IP, immunoprecipitation with antibody to FLAG-tagged HMO1. (B) Densitometric semi-quantitative analysis of ChIP data shown in (A), obtained with ImageJ software. (C) ChIP showing HMO1 localization at indicated loci relative to the DSB site after DNA damage (galactose) and during repair (glucose). (D) Densitometric analysis of ChIP data shown in (C). Fold enrichment = ChIP/Input DNA. Three independent experiments were performed. Error bars represent standard deviation.