Table 2. Efficiencies of transcription termination at the λ tR2 terminator by wild-type and mutant RNAPs.
RNAP | tR2 (%) | Rel. |
---|---|---|
WT | 50 ± 1 | 1 |
D446A | 34 ± 1 | 0.68 |
E546A | 41 ± 3 | 0.82 |
W183A | 53 ± 6 | 1.1 |
Δ443–451 | 52 ± 5 | 1.0 |
Δ533–546 | 69 ± 3 | 1.4 |
The last column shows termination efficiencies relative to wild-type RNAP.