Table 3. HisP half-life times (in seconds) for wild-type and mutant RNAP variants.

RNAP	hisP WT	hisP +1C	hisP +2C
WT	64.0 ± 6.4	11.7 ± 1.9	87.0 ± 1.7
	1	0.18	1.4
D446A	24.8 ± 1.7	13.9 ± 2.1	36.1 ± 5.5
	0.39	0.22	0.56
E546A	24.5 ± 3.1	8.2 ± 1.1	35.1 ± 3.8
	0.38	0.13	0.55

The numbers in bold show the t_1/2 values relative to the value measured for wild-type RNAP on the wild-type hisP template.