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. 2015 May 20;43(12):5824–5837. doi: 10.1093/nar/gkv507

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — TRF1-Myb and TRF2-Myb bind to nucleosomal telomeric sequences with similar affinities. (A, C) Gel mobility-shift assay. Naked DNA (lanes 1–7) and NCPs formed on Tel8-L (lanes 8–14) were incubated with increasing amounts of TRF1-Myb (A) or TRF2-Myb (C). The molar concentration of TRF1-Myb and TRF2-Myb is indicated above the lanes. Samples were separated on a 4.5% polyacrylamide gel. (B, D) Percentages of unbound DNA and NCPs are reported as a function of protein concentration in the reaction. The concentration of TRF1-Myb (B) and TRF2-Myb (D) at which 50% of naked DNA and nucleosome remain unbound has been extrapolated.