Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 20;43(12):5824–5837. doi: 10.1093/nar/gkv507

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Nucleosome influences binding of TRF1 and TRF2 to adjacent DNA. (A, C) Gel mobility-shift assay. Naked DNA (lanes 1–5) and NCPs formed on Tel2-601-Tel2 (lanes 6–10) were incubated with increasing amounts of TRF1 (A) or TRF2 (C). The molar concentration of TRF1 and TRF2 are indicated above the lanes. Samples were separated on a 0.8% agarose gel. (B, D) Percentages of unbound DNA and NCPs are reported as a function of protein concentration in the reaction. The concentration of TRF1 (B) and TRF2 (D) at which 50% of naked DNA and nucleosome remain unbound has been extrapolated.