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. 2015 May 20;43(12):5824–5837. doi: 10.1093/nar/gkv507

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — TRF1 alters telomeric nucleosome spacing in vitro. MNase digestion of chromatin assembled on the 601-Tel DNA fragment. (A) Lane 1, 100-bp DNA ladder; lane 2, chromatin assembled in the absence of TRF1; lane 3, chromatin assembled in the presence of 70-nM TRF1; lane 4, chromatin assembled in the presence of 200-nM TRF1; (B) Lane 1, 100-bp DNA ladder; lane 2, chromatin assembled in the absence of TRF1; lane 3, chromatin assembled in the presence of 70-nM TRF1. All samples are digested with 400 U/ml of MNase. A schematic drawing of the nucleosomal positioning and spacing is represented on the right.