Figure 7.

Effect of the partner site on resolvase-mediated cleavage. (A) 50 bp n-site I, 5′-³²P-labelled at the left end (20 nM), was treated with NM resolvase (800 nM) in the presence of excess (80 nM) unlabelled site I (black points and line) or n-site I (blue points and line). Aliquots were stopped at various time points and cleavage was quantified following gel electrophoresis. (B) 50 bp site I, 5′-³²P-labelled at the left end, was mixed with excess unlabelled site I or n-site I and treated with NM resolvase. Cleavage was quantified as in part (A), except that the points represent the sum of cleavage and recombinant products. Data in parts (A) and (B) were from triplicate experiments, as described in the legend to Figure 4B.