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. 2015 May 26;43(12):6049–6061. doi: 10.1093/nar/gkv546

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Cells producing unit-sized crRNA through transcription termination interfere with plasmid transformation. Efficiency of transformation (EOT) of three plasmids—pG8 containing a wild-type g8 protospacer and a functional PAM (blue bars), pG8mut, containing g8 protospacer with a C to T substitution at position +1 (yellow bars), and the pT7 blue vector (gray bars)—into indicated induced cells is presented. The height of the bars represents mean value obtained in three independent experiments and standard deviations are shown.