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. 2015 May 30;43(12):5687–5698. doi: 10.1093/nar/gkv566

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Tricine-SDS-PAGE analyses of single D-aa incorporation with wild-type EF-Tu and EF-Tu N273S. Template ‘G₁’ was translated in the presence of tRNA^Gly_u misacylated with selected d- and l-amino acids. (A) Autoradiograms of the gels. (B) Quantification of full-length product bands relative to bands from l-reactions with wild-type EF-Tu (lane 2 in (A)). EF-Tu N273S is inferior to the wild-type in all cases.