Table 2.
List of Hydrogen bonds between protein and substrates and conservation in MltA
| Sugar Site | Ligand Atom | +NAG (4wjt) | +Anhydro (4wlk) | MltA (2pi8) | Conserved YuiC vs MltA |
|---|---|---|---|---|---|
| −2 | O1 | Thr152 O via HOH A550 | None | (O4 of −1) Gln162 O | No. Thr100 YuiC is closest to MltA Gln162 |
| −2 | O3 | Leu111 O | Leu111 O | None | No |
| −2 | O3 | Lys102 NZ | Lys102 NZ | No | |
| −2 | O4 | HOH A430/435 | None | None | |
| −2 | O5 | Thr100 O via HOH A531 | HOH416 is too far (3.7 Å) | None | |
| −2 | O6 | Ser154 N and OG via HOH 539 | None | None | |
| −2 | O7 | Lys102 NZ | Lys102 NZ | ||
| −2 | O7 | Gly110 N and Ala 95 O via HOH A489 | Gly110 N and Ala 95 O via HOH A428 | Ser164 OG | No. HOH 428 and Ser164 OG are close |
| −2 | N2 | Asp151 OD1 and OD2 | Asp151 OD1 and OD2 | Asp297 OD2 | Yes. Asp151/297 |
| −1 | O1 | Asp162 OD2 | (O4 of +1) none but Asp308 mutated to Ala | Yes. Asp162/308 | |
| −1 | O3 | None | None | ||
| −1 | O5 | None | None | ||
| −1 | O6 | Val298 O | |||
| −1 | O7 | Ser 154 N and OG Ala 155 N | Gly300 N and Ala 301 N via HOH A1045 | Yes, but contact longer via water in MltA | |
| −1 | N2 | Ser99 O | Val161 O | No. Close in space but not conserved |