Fig. 3.

Critical weight determination of wild-type and black mutant larvae on rapamycin-treated diets. a, b Critical weight determination of wild-type (a) and black mutant larvae (b) on DMSO- or rapamycin-treated diets. The x-axis indicates the weight at which the animals were switched from the nutritive diet to the non-nutritive diet, and the y-axis represents the time from starvation to gut purge (measured in days). Black and blue lines represent animals fed a DMSO- or a rapamycin-treated diet, respectively. The solid lines and closed symbols represent larvae that were not starved, and the dotted lines and open symbols represent starved animals. Student’s t-test was used to compare the means for each weight category. *denotes P < 0.05; **denotes P < 0.001; ***denotes P < 0.0001. c The terminal growth period of wild-type and black mutant larvae on DMSO- or rapamycin-treated diets. Error bars represent standard errors