Table 5.
MMPs enzymatic assay of cell medium un-treated (CTR) and treated (H-HA0.1 %, L-HA0.1 %, H-HA/L-HA 0.1 % and 1 %) after 24 of incubation with a scratched HaCat monolayer. The assay was performed for 6 h under stirring at 37 °C. The reduction percentage was calculated as [(Gnt0- Gnt6h)/Gnt0]*100 where Gnt0 is gelatin amount at initial time and Gnt6h is gelatin amount after 6 h incubation time
Sample | Reduction percentage (%) |
---|---|
Gn + cell medium(CTR) | 50,9 |
Gn + cell medium(H-HA 0,1 %) | 57,6 |
Gn + cell medium(L-HA 0,1 %) | 56,9 |
Gn + cell medium(H-HA/L-HA 0,1 %) | 59,8 |
Gn + cell medium(H-HA/L-HA 1 %) | 64,4 |