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. Author manuscript; available in PMC: 2015 Jul 11.
Published in final edited form as: Nature. 2014 Jul 2;513(7516):110–114. doi: 10.1038/nature13441

Figure 1. IDH mutant alleles block hepatocyte differentiation.

Figure 1

a–d. Hepatoblasts (HBs) expressing empty vector (EV) or the indicated IDH alleles at physiologic levels (see Extended Data Fig. 1a), were transferred to uncoated plates to induce hepatocyte differentiation. a, Hepatocyte sphere formation. b, proliferation, c, hepatocyte marker expression (qRT-PCR). d, Heat map of hepatocyte and biliary gene expression.

e,f. HB cells treated with 2.5µM AGI-5027 or DMSO vehicle. e, hepatocyte sphere formation (upper panel), proliferation (lower panel). f, hepatocyte marker expression.

g. HB cells in matrigel assessed for biliary differentiation. Tubular structures/6 cm dish ± std. are quantified. *P<0.05. Scale bars, 100 µm.