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A) MBL-catalyzed β-lactam hydrolysis. B) Site-specific labeling of M67C NDM-1 with 3-bromo-1,1,1-trifluoroacetone (BFA) to give NDM-1*. 19F NMR spectra of NDM-1*-di-ZnII complex, apo-NDM-1*, and denatured NDM-1* (obtained by incubation with 2 M guanidinium chloride) revealed distinctive signal pattern.
