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. Author manuscript; available in PMC: 2016 Jul 2.
Published in final edited form as: Mol Cell. 2015 Jun 11;59(1):62–74. doi: 10.1016/j.molcel.2015.05.020

Figure 5. Samd14 Upregulates c-Kit Expression.

Figure 5

(A) RNA-seq of c-Kit, cd47, Tfrc, Epor, and Flt3 mRNA in R1 fetal liver cells. (B) CD71low, Ter119− cells (R1 cells) were sorted from fetal liver cells 72 h post-expansion, stained with anti-c-Kit and anti-CD71 antibodies, and MFI quantitated. (C) Kit mRNA and primary transcript analysis in R1 cells. (D) Western blot analysis of c-Kit in control and Samd14-knockdown R1 cells. (E) c-Kit and CD71 surface protein analyzed by Sulfo-NHS-Biotin conjugation of surface proteins in live cells, followed by streptavidin pulldown/Western blotting. Statistical significance: mean +/− SEM.; *, p<0.05; **, p<0.01; ***, p<0.001.