qPCR analyses of Mstn (a), Myocd (b), Mdfi (c), and Mef2d (d) expression in HL1 and Sol8 cells, respectively, transfected with miR-27 and miR-125, as stated in the corresponding panel. Observe that overexpression of miR-27 leads to downregulation of Mstn and upregulation of Myocd in HL1 cells, but not in Sol8 cells, while miR-125 overexpression does not alter any of these genes. Importantly, miR-27 overexpression downregulates Mdfi in HL1 cells, while it is upregulated in Sol8 cells. In the case of miR-125 overexpression, a similar effect is observed for Mef2d but in reverse mode; that is, miR-125 upregulates Mef2d in HL1 cells and downregulates it in Sol8 cells (n = 3). *
P < 0.05, **
P < 0.01, and ***
P < 0.001.