Table 1.
List of the oligonucleotides sequences used in the qPCR assays. Note that all primers were designed using the Primer3 (http://biotools.umassmed.edu/bioapps/primer3_www.cgi) online tool, fixing the primer length to 100–200 nucleotides and an annealing temperature of 60°C. MgCl2 concentration was always the same since SSOFast EvaGreen Master mix was used in all qPCR experiments.
| Gapdh | Fw: 5′-TCTTGCTCAGTGTCCTTGCTGG-3′ | 180 pb |
| Rv: 5′-TCCTGGTATGACAATGAATACGC-3′ | ||
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| β-Actin | Fw: 5′-CCAGAGGCATACAGGGAC-3′ | 144 pb |
| Rv: 5′-TGAGGAGCACCCTGTGCT-3′ | ||
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| Myocd | Fw: 5′-TTTTCAATTCCATCCCCAAC-3′ | 210 pb |
| Rv: 5′-CCCAGGGATCTTTGGAATTT-3′ | ||
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| Mdfi | Fw: 5′-CAGGCTCTGAACAGCATTGA-3′ | 125 pb |
| Rv: 5′-GGTTCTGAGAGGTGGTCGTG-3′ | ||
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| Mstn | Fw: 5′-GGCTCTTTGGAAGATGACGA-3′ | 188 pb |
| Rv: 5′-GGAGTCTTGACGGGTCTGAG-3′ | ||
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| Runx1 | Fw: 5′-TACCTGGGATCCATCACCTC-3′ | 164 pb |
| Rv: 5′-GACGGCAGAGTAGGGAACTG-3′ | ||
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| Mef2c | Fw: 5′-GGGGTGAGTGCATAAGAGGAC-3′ | 288 pb |
| Rv: 5′-AGAAGAAACACGGGGACTATGGG-3′ | ||
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| Mef2d | Fw: 5′-TCTCCCAGTCTACCCACTCG-3′ | 162 pb |
| Rv: 5′-CAGGTGAACTGAAGGCTGGT-3′ | ||