Figure 6.

SmDC inhibited Th1 response and induced Treg response regardless of MTX in coculture with arthritogenic T cells. Arthritogenic T cells, collected from CIA mice 21 days after CII-inoculation, were cocultured with smDC in the presence or absence of MTX up to 5000 nM. (a) CD4⁺IFN-γ ⁺ Th1 cells were analyzed by FACS. (b) CD4⁺Foxp3⁺ Treg cells were analyzed by FACS. Statistical data are shown from 3 different mice as mean ± SEM. ^∗ p < 0.05, ^∗∗ p < 0.001 in Student's t-test.