Skip to main content
. 2015 Jun 28;2015:729509. doi: 10.1155/2015/729509

Figure 8.

Figure 8

Apocynin suppressed TMT-induced MAPKs activations, p-IKB, iNOS, NO production, and TNF-α release in BV-2 cells. (a)–(d) The treated cells were subjected to western blot analysis to assess the expression of phosphorylated p38 and JNK at 6 hr and p-IκBα and iNOS at 12 hr. The bar graphs represent the band intensity of each protein form normalized to total. For (e) and (f), the culture media of treated cells at 24 hr were collected and used for (e) NO production measurement by Griess reagent method and (f) TNF-α detection by using Elisa kit. The data are represented as mean ± SEM (n = 4–7) ∗ P < 0.05 compared with the control group; # P < 0.05 compared with the value of TMT.