Figure 4.

Effects of MEPT on the formations of acidic vesicular organelle (AVO) in HSC-4 cells. Cells were treated with vehicle or 25, 50, 100, or 150 μg/mL of MEPT for 24 h, then stained with acridine orange, and visualized by fluorescent microscopy. AVO staining at MEPT concentrations of (a) 0 μg/mL; (b) 25 μg/mL; (c) 50 μg/mL; (d) 100 μg/mL; (e) 150 μg/mL; and (f) Earle's balanced salt solution (EBSS, positive control for autophagy) (original magnification, ×400).