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Expression and activation of MAPK proteins in MEPT-treated HSC-4 cells. Western blot analysis was conducted to measure the total and phosphorylated forms of p38, ERK, and JNK in HSC-4 cells treated with 0, 25, 50, or 100 μg/mL of MEPT for 24 h or 10 μM TFP for 24 h. The expression levels of phospho-p38, phospho-ERK, and phospho-JNK were normalized using a loading control.
