Fig. 4. Effects of zerumbone on VEGFR2- and FGFR1-mediated signaling pathways. (A) HUVECs were treated with various concentrations of zerumbone and stimulated with human VEGF (10 ng/ml) for 15 min. Phosphorylation of VEGFR2 by VEGF was determined using Western blot. (B) HUVECs were treated with zerumbone and stimulated with human bFGF (50 ng/ml) for 15 min. Phosphorylation of of FGFR by bFGF were determined using Western blot. The bands were quantified using image analysis software and their relative intensity was expressed as fold against the image of the VEGF- or bFGF- stimulated cells. ^***p<0.001 versus VEGF or bFGF+0 µM zerumbone-treated cells.