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. 2015 Jul 13;6:512. doi: 10.3389/fpls.2015.00512

Figure 7.

Figure 7

HDAC activity of recombinant AhHDA1 produced in E. coli BL21. (A) SDS-PAGE showing (1) total protein from E. coli cells expressing the recombinant plasmid pPROEX-AhHDA1 before induction by IPTG; (2) total protein from E. coli cells expressing recombinant plasmid pPROEX-AhHDA1 after induction by IPTG; for 20 h; and (3) purified AhHDA1 protein. (B) In vitro HDAC activity assay of the recombinant AhHDA1 protein. 1, positive control, extract from Hela cells; 2, extract from Hela cells treated with 4 μM TSA; 3, negative control, extract form E. coli cells containing plasmid pPROEX; 4, extract from E. coli cells containing plasmid pPROEX-AhHDA1 without IPTG; 5, extract from cells containing plasmid pPROEX after induction by IPTG; 6, extract from cells containing plasmid pPROEX-AhHDA1 after induction by IPTG for 20 h; 7, extract from cells containing plasmid pPROEX-AhHDA1 after induction by IPTG, but treated with 4 μM TSA for 20 h; 8, purified recombinant AhHDA1 protein; 9, purified recombinant AhHDA1 protein treated with 4 μM TSA. Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.