FIG. 1.

Clonogenically culturing and expanding CD34+ liver cancer stem cells (LCSC). (A) CD34+ LCSC formed colonies with round and packed morphology after culturing on mouse embryonic fibroblast feeder cells under our defined condition, and multiple morphologies were observed on the feeder cells. (B) CD34+ LCSC could be cryopreserved and then recovered on feeder cells, and multiple morphologies were also shown similar to those before freezing. (C) CD34+ LCSC could be expanded on a commercial extracellular matrix (ECM) plate to remove feeder cells and maintained feeder free for a short period employing our culture conditions. CD34+ LCSC also maintained multiple morphologies on ECM. Scale bar, 100 μm. Color images available online at www.liebertpub.com/scd