Comparison of expression patterns of stem cell transcription factors among three cell types. (A) Expression of stem cell transcription factors (SOX2, OCT4, NAONG, c-Myc, Klf4, Lin28) and stem cell marker, CD34, was compared among the parental PLC (PLC), the uncloned CD34+ LCSC sorted from the parental PLC (sorted CD34+), and cloned CD34+ LCSC (cloned CD34+) employing quantitative polymerase chain reaction (qPCR). (B, C) Expression of these six stem cell transcription factors was evaluated and compared among the sorted CD34+ LCSC (sorted CD34+), the cloned CD34+ LCSC (cloned CD34+), and human embryonic stem cell using qPCR. All values are mean±standard error of the mean (SEM) from three independent experiments. *P<0.05; **P<0.01.