Analysis of drug resistance and in vitro differentiation. (A) The percentage of CD34+ cells on mouse embryonic fibroblasts feeder cell culture. (B) The percentage of CD34+ cells at day 3 on ECM after being moved from feeder cell culture. (C) The percentage of CD34+ cells at day 14 on ECM culture after being moved from feeder cell culture. (D) The percentage of CD34+ cells at day 14 on ECM culture with treatment by cisplatin at 2 μg/mL from day 7 after being moved from feeder cell culture. (E) Expression of liver genes [albumin (ALB), AFP, CK19, and CK7], liver cancer markers (CD133, EpCAM, CD44, and CD90), as well as CD31 and CD34 was evaluated by qPCR at days 4, 9, and 13 after the differentiation of cloned CD34+ LCSC and compared to undifferentiated cloned CD34+ LCSC (CD34+). All values are mean±SEM from three independent experiments. *P<0.05; **P<0.01.