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. 2015 Mar 9;24(13):1582–1590. doi: 10.1089/scd.2014.0538

FIG. 1.

FIG. 1.

Atherosclerosis (AS) reduced the proliferative and migratory activities of endothelial progenitor cells (EPCs). (A) Representative images showing lipid staining by Oil red O in the aortic trees obtained from normal diet (ND), 12-week high-fat diet (HFD), and 16-week HFD ApoE−/− mice. (B) The number of EPCs was counted under a microscope after 7 days in culture. The data are presented as the mean number of EPCs per high-power field (HPF)±SE. (C) The proliferative activity of EPCs was assessed using a WST-8 assay. The data are presented as the mean absorbance at 450 nM±SE. (D) EPC migration was evaluated by counting the number of EPCs on the bottom of a modified Boyden chamber membrane. The data are presented as the mean number of EPCs per HPF±SE. n=5, *P<0.05 versus ND-EPCs.