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. 2015 Mar 9;24(13):1582–1590. doi: 10.1089/scd.2014.0538

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 3. — Messenger RNA and protein expression of store-operated calcium channel (SOCC) components decreased in HFD-EPCs. (A) Stim1, Orai1, and TRPC1 mRNA levels were significantly reduced in HFD-EPCs compared with ND-EPCs by RT-qPCR. (B) Western blotting and densitometry analyses revealed significant reductions in the levels of Stim1, Orai1, and TRPC1 proteins in HFD-EPCs compared with ND-EPCs. The data are presented as the mean±SE, n=3; *P<0.05 versus ND-EPCs. RT-qPCR, reverse transcription quantitative real-time PCR.