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VEGF-induced eNOS phosphorylation was reduced in HFD-EPCs and in SOCE-inhibited EPCs. (A–C) Representative western blots for the detection of phospho (p)-eNOS and p-Akt in ND-EPCs (A), HFD-EPCs (B), and ND-EPCs+shStim1 (C) stimulated with VEGF. (D, E) Quantitative analyses of VEGF-induced eNOS and Akt phosphorylation normalized to total eNOS or Akt, respectively. n=3, *P<0.05 versus ND-EPCs.
