FIG. 7.
SOCE inhibition reduced the proliferation and migration of EPCs. (A) Representative time courses of [Ca2+]i changes showing that both specific inhibitors and shRNA-Stim1 inhibited SOCE in the ND-EPCs. ND=13, ND+2-APB=15, ND+ML-9=12, ND+shStim1=13. (B) Quantification of TG-induced SOCE amplitudes. ND=41, ND+2-APB=43, ND+ML-9=37, ND+shStim1=35. Cells in each group were harvested from three different mice. The data are presented as the mean±SE; *P<0.05 versus ND-EPCs. (C) The number of EPCs was counted under a microscope. (D) EPC proliferative activity was assessed by WST-8 assay. (E) EPC migration was evaluated by counting the number of EPCs on the bottom of a modified Boyden chamber membrane. (C–E) The data are presented as the mean±SE, n=5; *P<0.05 versus ND-EPCs.