FIG. 3.

PGCs in the in vitro cultured AGMs are Ki67 positive and increase in number during 3 days of culture. (A) Number of OG-positive PGCs in male and (B) female embryos at the indicated time points. (C) Immunofluorescence of in vitro cultured AGM from days 1–3 for Mvh-positive PGCs (red), Ki67 (green), and DAPI (blue). (D) Average number of OG-positive PGCs in cultured AGMs at days 1–3 of culture. All images were obtained by using at least n=3 independent biological replicates of gonads or AGMs.